Metagenomic analysis reveals functional genes in soil microbial electrochemical removal of tetracycline.

Microbial fuel cells (MFCs) are capable of removing tetracycline in soils, in which the degradation efficiency of tetracycline is hindered by its strong adsorption capacity. Phosphate was chosen as a competitor for tetracycline adsorption to improve its removal rate in soil MFCs. The results showed that 42-50% of tetracycline was degraded within 7 days, which was 42-67% higher than open-circuit treatments. Compared with closed-circuit treatments without phosphate addition, the removal efficiencies of tetracycline after phosphate addition increased by 19-25% on day 51, and accumulated charge outputs were enhanced by 31-52%, while the abundance of antibiotic resistance genes decreased by 19-27%. Like Geobacter, the abundance of Desulfurispora and Anaeroomyxobacter in the anode showed similar tendencies with current densities, suggesting their dominant roles in bioelectricity generation. Gemmatimonadetes bacterium SCN 70-22, Azohydromonas australica, Steroidobacter denitrificans and Gemmatirosa kalamazoonesis were found to be potential electrotrophic microbes in the cathode. The expressed flavoprotein 2,3-oxidoreductase, quinol oxidase and fumarate reductase might have promoted the transfer efficiency of electrons from cathodes to cells, which finally accelerated the biodegradation rate of tetracycline in addition to the polyphenol oxidase. This study provides an insight into functional enzyme genes in the soil microbial electrochemical remediation.

Characterization of Acid-Aged Biochar and its Ammonium Adsorption in an Aqueous Solution.

According to its characteristics, biochar originating originating from biomass is accepted as a multifunctional carbon material that supports a wide range of applications. With the successfully used in reducing nitrate and adsorbing ammonium, the mechanism of biochar for nitrogen fixation in long-term brought increasing attention. However, there is a lack of analysis of the NH4+-N adsorption capacity of biochar after aging treatments. In this study, four kinds of acid and oxidation treatments were used to simulate biochar aging conditions to determine the adsorption of NH4+-N by biochar under acidic aging conditions. According to the results, acid-aged biochar demonstrated an enhanced maximum NH4+-N adsorption capacity of peanut shell biochar (PBC) from 24.58 to 123.28 mg·g-1 after a H2O2 modification. After the characteristic analysis, the acid aging treatments, unlike normal chemical modification methods, did not significantly change the chemical properties of the biochar, and the functional groups and chemical bonds on the biochar surface were quite similar before and after the acid aging process. The increased NH4+-N sorption ability was mainly related to physical property changes, such as increasing surface area and porosity. During the NH4+ sorption process, the N-containing functional groups on the biochar surface changed from pyrrolic nitrogen to pyridinic nitrogen, which showed that the adsorption on the surface of the aged biochar was mainly chemical adsorption due to the combination of π-π bonds in the sp2 hybrid orbital and a hydrogen bonding effect. Therefore, this research establishes a theoretical basis for the agricultural use of aged biochar.

Investigation of watershed nutrient export affected by extreme events and the corresponding sampling frequency.

Although the real-time monitoring technique has been widely applied due to the improvement of sensors, development of traditional sampling methods is still worth of being discussed due to the economically feasibility. Currently, extreme events (e.g. extreme rainfall caused by climate change) play a relatively important role in nutrient export. However, impacts of extreme events on the optimization of sampling strategy is still not well addressed despite the uncertainty of different frequency sampling programs has been sufficiently discussed in previous studies. Therefore, the corresponding impact of extreme events impact on the optimization of sampling strategy was investigated by examining temporal (i.e., inter-annual and seasonal) variations of available data. Uncertainty of nutrient flux estimates under different sampling frequencies was explored by subsampling daily monitoring data. Results showed that uncertainty in flux estimates differed between nitrogen and phosphorus. The relative error (RE) in annual TN flux estimates ranged from -4.2% to 2.4% (once per three-day) to -21.4-31.1% (monthly sampling), while the RE in annual TP flux estimates varied from -14.1% to 8.2% (once per three-day) to -65.9%-163.4% (monthly sampling). Biweekly and weekly sampling routines are considered the optimal sampling program for total nitrogen (TN) and for total phosphorus (TP) when the extreme events impact were not been considered. The uncertainty of flux estimates with different sampling frequencies increased with the increasing extreme events. High level of uncertainty occurred in years with the most extreme events in 2012 (RE: 21.4-69.0% for TN, 33.3-96.6% for TP), while the lowest can be found in 2011 (RE: 0-20.7% for TN, 0-48.3% for TP) (with the fewest extreme events). In addition, uncertainty in TN and TP flux estimates was generally greater during summer season than during other seasons. These results highlighted the important role of extreme events in nutrient export. Approximately half of the annual TN and TP flux occurred in some extreme days that only accounted for less than 20% in the same year. The onset of these extremes of nutrient export was likely due to the stormflow with addition of external fertilizer and the direct discharge of surface ponding water from paddy fields during special periods of time. These results would be helpful for the optimization of sampling strategy.

Shifting interactions among bacteria, fungi and archaea enhance removal of antibiotics and antibiotic resistance genes in the soil bioelectrochemical remediation.

BACKGROUND: Antibiotics and antibiotic resistance genes (ARGs) are two pollutants in soil, especially ARGs as one of the top three threats to human health. The performance of soil microbial fuel cells (MFCs) fuelled with antibiotics was investigated. RESULTS: In this study, soil MFCs spiked with tetracycline exhibited optimal bioelectricity generation, which was 25% and 733% higher than those of MFCs spiked with sulfadiazine and control, respectively. Compared with the non-electrode treatment, not only did functional micro-organisms change in open- and closed-circuit treatments, but also the microbial affinities, respectively, increased by 50% and 340% to adapt to higher removal of antibiotics. For the open-circuit treatment, the ineffective interspecific relation of micro-organisms was reduced to assist the removal efficiency of antibiotics by 7-27%. For the closed-circuit treatment, an intensive metabolic network capable of bioelectricity generation, degradation and nitrogen transformation was established, which led to 10-35% higher removal of antibiotics. Importantly, the abundances of ARGs and mobile genetic element (MGE) genes decreased after the introduction of electrodes; especially in the closed-circuit treatment, the highest reduction of 47% and 53% was observed, respectively. CONCLUSIONS: Soil MFCs possess advantages for the elimination of antibiotics and ARGs with sevenfold to eightfold higher electricity generation than that of the control treatment. Compared with sulphonamides, the enhancement removal of tetracycline is higher, while both potential ARG propagation risk is reduced in soil MFCs. This study firstly synchronously reveals the relationships among bacteria, fungi and archaea and with ARGs and MGE genes in soil bioelectrochemical systems.

Bioelectrochemical removal of tetracycline from four typical soils in China: A performance assessment.

Exposure to tetracycline in soil causes microbial mutations. Soil microbial fuel cells (MFCs) can promote the degradation efficiency of contaminants while generating bioelectricity under anaerobic conditions. MFC performance varies amongst different types of soils due to distinctive soil properties. This study assesses the performance of soil MFCs filled with four typical Chinese soils and explores key factors regulating bioelectricity generation and tetracycline degradation. Except for the MFCs filled with black soil, tetracycline degradation rates improved in soil MFCs, particularly in those filled with Chao soil, which enhanced the degradation rate by 39% relative to the corresponding control. Additionally, soil MFCs filled with Chao soil exhibited the highest charge output of 1347 ±â€¯357C, which was 100-499% higher than that of MFCs with other soils. According to redundancy analysis, soil particle size, pH, conductivity and dissolved organic carbon content showed positive association with tetracycline degradation and charge output, while the adsorption of tetracycline had a negative association with degradation rate. Thus, the adsorption of tetracycline restricted its removal efficiency in soil MFCs, and high soil conductivity and large particle size promoted electron transfer, enhancing biocurrent intensity, which increased tetracycline degradation efficiency.

Contrasting impacts of long-term application of manure and crop straw on residual nitrate-N along the soil profile in the North China Plain.

The effects of long-term animal manure application and crop straw incorporation on the migration of carbon (C) and nitrogen (N) deep into the soil profile and the associated N leaching risk in particular have not been thoroughly elucidated to date. Soil profile samples were collected from depths of up to 200 cm from the following four treatments in a 27-year field experiment on the North China Plain: N + phosphorus (P) + potassium (K) fertilizers (NPK), NPK + 22.5 t ha-1 swine manure (NPKM), NPK + 33.7 t ha-1 swine manure (NPKM+) and NPK + straw incorporation (NPKS). The results revealed that long-term manure application and straw incorporation significantly enhanced the soil organic C (SOC) and total N (TN) contents in the upper 20 cm and that this effect was weaker in the deeper soil layers (P < 0.05). Residual nitrate-N (NO3--N) contents at 0 to 40 cm and 120 to 200 cm in the NPKM and NPKM+ were 4-16 and 2-9 times higher than those in the NPK and NPKS, respectively. These results indicated a greater potential for N leaching from manure addition and a higher propensity for NO3--N leaching out of the 40-100 cm soil layer. Pearson relationship analysis demonstrated that NO3--N content was clearly affected by SOC and dissolved organic N (DON) contents along the soil profile (20-200 cm), implying that the higher residual NO3--N contents in the deeper soil from manure addition were partially attributable to the mineralization and nitrification of the downward SOC and DON. Interestingly, a low level of residual NO3--N combined with negative mineralization in the 120-200 cm soil layers of the NPKS treatment was observed, suggesting that straw incorporation promotes soil NO3--N retention. Thus, we concluded that long-term manure application is beneficial for soil NO3--N content retention, whereas long-term straw incorporation benefits NO3--N retention.

[Impact of Human Activities on Net Anthropogenic Nitrogen Inputs (NANI) at Township Scale in Erhai Lake Basin].

Excessive nitrogen inputs from human activities have become the main cause of water eutrophication and related ecological hazards. In order to study the impact of human activities on nitrogen in the basin, and based on statistical data of administrative units in 16 towns and villages, this study used the NANI model to calculate net anthropogenic nitrogen inputs (NANI) at township scale in Erhai Lake basin. Results show that the total amount of NANI in Erhai Lake basin was 29.81×103 t in 2014, and nitrogen input intensity per unit area was 10986 kg·(km2·a)-1, significantly higher than the national average. The input of nitrogen from food by the local tourist population was 0.26×103 t, accounting for 8% of local food nitrogen input. Nitrogen input from chemical fertilizer is the largest NANI input source, accounting for 47% of net nitrogen input in the basin, followed by net nitrogen input of food and feed. The spatial distribution of NANI at township scale shows evident regionalization, with higher values in the north and lower values in the south of the basin. The intensity of NANI in towns with cropland or population is high. The corresponding risk of nitrogen pollution in Erhai Lake basin is therefore a primary concern, and will remain so in the near future.

Influences of agricultural land use composition and distribution on nitrogen export from a subtropical watershed in China.

Despite the significant impacts of agricultural land on nonpoint source (NPS) nitrogen (N) pollution, little is known about the influence of the distribution and composition of different agricultural land uses on N export at the watershed scale. We used the Soil and Water Assessment Tool (SWAT) to quantify how agricultural distribution (i.e., the spatial distributions of agricultural land uses) and composition (i.e., the relative percentages of different types of agricultural land uses) influenced N export from a Chinese subtropical watershed, accounting for aquatic N retention by river networks. Nitrogen sources displayed high spatial variability, with 40.7% of the total N (TN) export from the watershed as a whole derived from several subwatersheds that accounted for only 18% of the watershed area. These subwatersheds were all located close to the watershed mouth. Agricultural composition strongly affected inputs to the river network. The percentages of dry agricultural land and rice paddy fields, and the number of cattle together explained 70.5% of the variability of the TN input to the river network among different subwatersheds. Total N loading to the river network was positively correlated with the percentage of dry land in total land areas and the number of cattle within subwatersheds, but negatively with the proportion of paddy fields. Distribution of agricultural land uses also affected N export at the mouth of the watershed. Moreover, N retention in the river network increased with increasing N transport distance from source subwatershed to the watershed mouth. Results provide important information to support improved planning of agricultural land uses at the watershed scale that reduces NPS nutrient pollution.

Antibiotic residues in liquid manure from swine feedlot and their effects on nearby groundwater in regions of North China.

A survey was conducted in regions of North China to better understand the effect of antibiotic residue pollution from swine feedlots to nearby groundwater environment. A total of nine experimental sites located in the regions of Beijing, Hebei, and Tianjin were selected to analyze the presence of residues of 11 most commonly used antibiotics, including tetracyclines (TCs), fluoroquinolones (FQNs), sulfonamides (SAs), macrolides, and fenicols, by using liquid chromatography spectrometry. The three most common antibiotics were TCs, FQNs, and SAs, with mean concentrations of 416.4, 228.8, and 442.4 µg L-1 in wastewater samples; 19.9, 11.8, and 0.3 µg L-1 in groundwater samples from swine feedlots; and 29.7, 14.0, and 0 µg L-1 in groundwater samples from villages. Ordination analysis revealed that the composition and distribution of antibiotics and antibiotic resistance genes (AGRs) were similar in groundwater samples from swine feedlots and villages. FQNs and TCs occurred along the path from wastewater to groundwater at high concentrations and showed correlations with ARGs, with a strong correlation between FQN resistance gene (qnrA) copy number. FQN concentration was also found (P < 0.01) in wastewater and groundwater in villages (P < 0.01). Therefore, antibiotics discharged from swine feedlots through wastewater could disseminate into surrounding groundwater environments together with ARG occurrence (i.e., qnrA, sulI, sulII, tetG, tetM, and tetO). Overall, this study suggests that the spread of veterinary antibiotics from swine feedlots to groundwater environments should be highly attended and controlled by restricting excess antibiotic usage or improving the technology of manure management.

[Seasonal Changes of the Pathways of Nitrogen Export from an Agricultural Watershed in China].

Nonpoint source pollution has become a major factor influencing the water quality. Identifying the pathway of nitrogen (N) transport from the source to the watershed mouth is a critical step in taking measures to control this pollution. However, it is difficult to identify the pathway of N transport because the transport pathway varies among different watersheds depending on the difference in the terrain, hydrology, and land cover etc and changes over time. Additionally, there is little knowledge about the major pathway of N transport through agricultural watersheds in the Yunnan Plateau lake area. The pathways of N export and their temporal variations over time were investigated in this study based on a typical agriculture-dominated watershed in a plateau lake area, Yunnan Province, and two-year monitoring data (June 2011-May 2013) in combination with a base flow separation program. The results show that the base flow accounts for most of the streamflow discharge (80.0%) and N export (71.1%). The proportion of the stream flow discharge via storm flow increases significantly with increasing rainfall. Therefore, the percentage of total N (TN) export via storm flow increases with increasing storm flow, which is closely related to rainfall. The major pathway of N export shifts toward storm flow when the storm flow proportion of the stream flow discharge increases up to 40%. During the monitoring period, the proportion of the TN export via storm flow increases up to 65.6% in the rainy season. This study provides important information for the improvement of the management of nonpoint source pollution at the watershed scale.

Optimizing the nitrogen application rate for maize and wheat based on yield and environment on the Northern China Plain.

Optimizing the nitrogen (N) application rate can increase crop yield while reducing the environmental risks. However, the optimal N rates vary substantially when different targets such as maximum yield or maximum economic benefit are considered. Taking the wheat-maize rotation cropping system on the North China Plain as a case study, we quantified the variation of N application rates when targeting constraints on yield, economic performance, N uptake and N utilization, by conducting field experiments between 2011 and 2013. Results showed that the optimal N application rate was highest when targeting N uptake (240kgha-1 for maize, and 326kgha-1 for wheat), followed by crop yield (208kgha-1 for maize, and 277kgha-1 for wheat) and economic income (191kgha-1 for maize, and 253kgha-1 for wheat). If environmental costs were considered, the optimal N application rates were further reduced by 20-30% compared to those when targeting maximum economic income. However, the optimal N rate, with environmental cost included, may result in soil nutrient mining under maize, and an extra input of 43kgNha-1 was needed to make the soil N balanced and maintain soil fertility in the long term. To obtain a win-win situation for both yield and environment, the optimal N rate should be controlled at 179kgha-1 for maize, which could achieve above 99.5% of maximum yield and have a favorable N balance, and at 202kgha-1 for wheat to achieve 97.4% of maximum yield, which was about 20kgNha-1 higher than that when N surplus was nil. Although these optimal N rates vary on spatial and temporal scales, they are still effective for the North China Plain where 32% of China's total maize and 45% of China's total wheat are produced. More experiments are still needed to determine the optimal N application rates in other regions. Use of these different optimal N rates would contribute to improving the sustainability of agricultural development in China.

[Characteristics of Nitrogen Variation and Its Response to Rainfall:A Case Study in Wuxi Port at Taihu Lake Basin].

Based on monitoring data of the water quality in the Wuxi port estuary of Taihu Lake from 2010 to 2015, we studied the temporal variation characteristics of nitrogen and its response to rainfall in Wuxi port. The results showed that the pollution level in Wuxi port is serious, with an average annual total nitrogen (TN) concentration of (4.41-5.92 mg·L-1), worse than the water environment quality grade Ⅴ standard (2 mg·L-1). The ammonia nitrogen (NH4+-N) concentration was 1.09-1.72 mg·L-1. The concentrations of TN and NH4+-N showed obvious seasonal variations, with the concentration of TN and NH4+-N in summer and autumn higher than in spring and winter. The concentration of TN in 2015, 2012, and 2011 was 5.92, 5.82, and 5.47 mg·L-1, respectively, which was significantly higher than in 2013 and 2014. The concentration of NH4+-N in 2011 (1.72 mg·L-1) was higher than in 2013 and 2015. With the increase of rainfall intensity, the TN concentration showed an increase after the first downward trend and then a decrease, while the NH4+-N concentration increased first and then decreased. In addition, the nitrogen concentration in the non-flood season was higher than in the flood season.

[Characteristics of Nitrogen and Phosphorus Emissions in the Gufu River Small Watershed of the Three Georges Reservoir Area].

To study the seasonal change characteristics and form composition of nitrogen and phosphorus output concentration and pollutant discharge load with rainfall characteristics at the outlet of Gufu river small watershed,the quality and quantity of water combined with natural rainfall events were monitored from January to December 2014.The results showed that the annual runoff volume of the Gufu river small watershed was 0.6×108 m3.The runoff was concentrated in raining season (from July to September),accounting for 63.9%.There was significant (P<0.01) positive correlation between the runoff flow and the annual rainfall.The annual emission of total nitrogen (TN) was 1432 t·a-1,and the emission was 853 t·a-1 during the raining season,accounting for 59.6% of annual TN emission content.The dissolved nitrogen was the main form of nitrogen emission,and the emission load of each month accounted for 55.4%-91.3% of TN.The positive correlation between the nitrate nitrogen concentration and rainfall was significant (P<0.05).The annual emission of total phosphorus (TP) was 563.1 t·a-1,and the content during the raining season accounted for 78.6% of TP annual emission content.The particle phosphorus (PP) was the main form of phosphorus emission,and the emission load of each month accounted for 41.9%-79.5% of TP.There was significant (P<0.01) positive correlation between the annual rainfall,sediment and TP,PP.The correlation between the total dissolved phosphorus concentration and rainfall was significant (P<0.05).

[Analysis of Spatial Variability of Water Quality and Pollution Sources in Lihe River Watershed, Taihu Lake Basin].

The source of pollutants in the Tai Lake basin and the characteristics of spatiotemporal variations were studied by conducting water quality monitoring in the Lihe River watershed to the west of Tai Lake in 2014. The dynamic changes in total phosphorus (TP), ammonia nitrogen (NH4+-N), and chemical oxygen demand (COD) were studied in flood and non-flood seasons at five monitoring points from upstream to downstream. The average concentrations of TP, NH4+-N, and COD were 0.176 mg·L-1, 1.075 mg·L-1, and 10.626 mg·L-1 respectively, and the water quality was lower than the grade Ⅳ standard. From upstream to downstream, the concentrations of TP and NH4+-N gradually increased. The water quality downstream was poor, worse than the gradeⅤstandard; however, COD was low and met the grade Ⅳ standard. During the non-flood season, the pollutant concentrations gradually increased from upstream to downstream. There was no obvious trend in flood season. Concentrations of pollutants gradually increased with the increase in the area of inhabited land and decrease in the area of forest land. The population density, livestock, and poultry production were significantly correlated with the concentrations of pollutants in the river. The pollutants in the Lihe River watershed mainly originated from human activities, and livestock and poultry breeding activities.

Row Ratios of Intercropping Maize and Soybean Can Affect Agronomic Efficiency of the System and Subsequent Wheat.

Intercropping is regarded as an important agricultural practice to improve crop production and environmental quality in the regions with intensive agricultural production, e.g., northern China. To optimize agronomic advantage of maize (Zea mays L.) and soybean (Glycine max L.) intercropping system compared to monoculture of maize, two sequential experiments were conducted. Experiment 1 was to screening the optimal cropping system in summer that had the highest yields and economic benefits, and Experiment 2 was to identify the optimum row ratio of the intercrops selected from Experiment 1. Results of Experiment 1 showed that maize intercropping with soybean (maize || soybean) was the optimal cropping system in summer. Compared to conventional monoculture of maize, maize || soybean had significant advantage in yield, economy, land utilization ratio and reducing soil nitrate nitrogen (N) accumulation, as well as better residual effect on the subsequent wheat (Triticum aestivum L.) crop. Experiment 2 showed that intercropping systems reduced use of N fertilizer per unit land area and increased relative biomass of intercropped maize, due to promoted photosynthetic efficiency of border rows and N utilization during symbiotic period. Intercropping advantage began to emerge at tasseling stage after N topdressing for maize. Among all treatments with different row ratios, alternating four maize rows with six soybean rows (4M:6S) had the largest land equivalent ratio (1.30), total N accumulation in crops (258 kg ha(-1)), and economic benefit (3,408 USD ha(-1)). Compared to maize monoculture, 4M:6S had significantly lower nitrate-N accumulation in soil both after harvest of maize and after harvest of the subsequent wheat, but it did not decrease yield of wheat. The most important advantage of 4M:6S was to increase biomass of intercropped maize and soybean, which further led to the increase of total N accumulation by crops as well as economic benefit. In conclusion, alternating four maize rows with six soybean rows was the optimum row ratio in maize || soybean system, though this needs to be further confirmed by pluri-annual trials.

Identifying critical nitrogen application rate for maize yield and nitrate leaching in a Haplic Luvisol soil using the DNDC model.

Identification of critical nitrogen (N) application rate can provide management supports for ensuring grain yield and reducing amount of nitrate leaching to ground water. A five-year (2008-2012) field lysimeter (1 m × 2 m × 1.2 m) experiment with three N treatments (0, 180 and 240 kg Nha(-1)) was conducted to quantify maize yields and amount of nitrate leaching from a Haplic Luvisol soil in the North China Plain. The experimental data were used to calibrate and validate the process-based model of Denitrification-Decomposition (DNDC). After this, the model was used to simulate maize yield production and amount of nitrate leaching under a series of N application rates and to identify critical N application rate based on acceptable yield and amount of nitrate leaching for this cropping system. The results of model calibration and validation indicated that the model could correctly simulate maize yield and amount of nitrate leaching, with satisfactory values of RMSE-observation standard deviation ratio, model efficiency and determination coefficient. The model simulations confirmed the measurements that N application increased maize yield compared with the control, but the high N rate (240 kg Nha(-1)) did not produce more yield than the low one (120 kg Nha(-1)), and that the amount of nitrate leaching increased with increasing N application rate. The simulation results suggested that the optimal N application rate was in a range between 150 and 240 kg ha(-1), which would keep the amount of nitrate leaching below 18.4 kg NO3(-)-Nha(-1) and meanwhile maintain acceptable maize yield above 9410 kg ha(-1). Furthermore, 180 kg Nha(-1) produced the highest yields (9837 kg ha(-1)) and comparatively lower amount of nitrate leaching (10.0 kg NO3(-)-Nha(-1)). This study will provide a valuable reference for determining optimal N application rate (or range) in other crop systems and regions in China.

Effects of feedstock and pyrolysis temperature on biochar adsorption of ammonium and nitrate.

Biochar produced by pyrolysis of biomass can be used to counter nitrogen (N) pollution. The present study investigated the effects of feedstock and temperature on characteristics of biochars and their adsorption ability for ammonium N (NH4(+)-N) and nitrate N (NO3(-)-N). Twelve biochars were produced from wheat-straw (W-BC), corn-straw (C-BC) and peanut-shell (P-BC) at pyrolysis temperatures of 400, 500, 600 and 700°C. Biochar physical and chemical properties were determined and the biochars were used for N sorption experiments. The results showed that biochar yield and contents of N, hydrogen and oxygen decreased as pyrolysis temperature increased from 400°C to 700°C, whereas contents of ash, pH and carbon increased with greater pyrolysis temperature. All biochars could sorb substantial amounts of NH4(+)-N, and the sorption characteristics were well fitted to the Freundlich isotherm model. The ability of biochars to adsorb NH4(+)-N followed: C-BC>P-BC>W-BC, and the adsorption amount decreased with higher pyrolysis temperature. The ability of C-BC to sorb NH4(+)-N was the highest because it had the largest cation exchange capacity (CEC) among all biochars (e.g., C-BC400 with a CEC of 38.3 cmol kg(-1) adsorbed 2.3 mg NH4(+)-N g(-1) in solutions with 50 mg NH4(+) L(-1)). Compared with NH4(+)-N, none of NO3(-)-N was adsorbed to biochars at different NO3(-) concentrations. Instead, some NO3(-)-N was even released from the biochar materials. We conclude that biochars can be used under conditions where NH4(+)-N (or NH3) pollution is a concern, but further research is needed in terms of applying biochars to reduce NO3(-)-N pollution.

Simultaneous heterotrophic nitrification and aerobic denitrification by bacterium Rhodococcus sp. CPZ24.

Rhodococcus sp. CPZ24 was isolated from swine wastewater and identified. Batch (0.25 L flask) experiments of nitrogen removal under aerobic growth conditions showed complete removal of 50 mg L(-1) ammonium nitrogen within 20 h, while nitrate nitrogen removal reached 67%. A bioreactor (50 L) was used to further assess the heterotrophic nitrification and aerobic denitrification abilities of Rhodococcus sp. CPZ24. The results showed that 85% of the ammonium nitrogen (100 mg L(-1)) was transformed to nitrification products (NO(3)(-)-N and NO(2)(-)-N) (13%), intracellular nitrogen (24%), and gaseous denitrification products (48%) within 25 h. The ammonium nitrogen removal rate was 3.4 mg L(-1)h(-1). The results indicate that the strain CPZ24 carries out simultaneous nitrification and denitrification, demonstrating a potential use of the strain for wastewater treatment.

[Effects of different irrigation modes in winter wheat growth season on the grain yield and water use efficiency of winter wheat-summer maize].

Three irrigation modes in winter wheat growth season were carried out in Heilonggang basin of North China Plain to investigate their effects on the grain yield, water consumption, and water use efficiency (WUE) of winter wheat-summer maize. The three irrigation modes included irrigation before sowing (75 mm, W1), irrigation before sowing and at jointing stage (75 mm + 90 mm, W2), and irrigation before sowing, at jointing stage, and at filling stage (75 mm + 90 mm + 60 mm, W3). With the irrigation modes W2 and W3, the increment of the annual yield of winter wheat-summer maize was 8.7% and 12.5% higher than that with W1, respectively. The water consumption in winter wheat growth season decreased with increasing irrigation amount, while that in summer maize growth season increased with the increasing irrigation amount in winter wheat growth season. The WUE of winter wheat with the irrigation mode W2 was 11.1% higher than that with W3, but the WUE of summer maize had less difference between irrigation modes W2 and W3. The annual WUE (WUE(T)) of W2 and W1 was 21.28 and 21.60 kg(-1) x mm x hm(-2), being 7.8% and 9.4% higher than that of W3, respectively. Considering the annual yield, water consumption, and WUE, irrigation mode W2 could be the advisable mode for water-saving and high-yielding.

[Potential of organic manures nutrient resources and their environmental risk in China].

Based on the data from China Agriculture Yearbook 2006 and other documents, the amounts of crop straws and of the excreta from human, livestock, and poultry in China in 2005 were estimated, with the potential and the environmental risk of their nutrient resources analyzed. In 2005, the total amount of crop straws in this country was 643 million tons, and that of the excreta from human, livestock, and poultry was 4625 million tons. The amounts of N, P2O5, and K2O from the straws and the excreta were 28.24, 12.82, and 29.48 million tons, being 1.08, 0.86, and 4.56 times of the inputs from chemical fertilizers in the same year, respectively. There was a great regional difference in the distribution of the organic manures nutrient resources. The total amount of N, P2O5, and K2O in the excreta was more than 4 million tons in Henan, Shandong, and Sichuan provinces, but less in Beijing, Tianjin, Shanghai, and northwest China, while the total amount of these nutrients in crop straws was more than 1.5 million tons in Henan and Shandong provinces, the main grain production areas, but less in northwest China. The per unit farmland load of N, P2O5, and K2O from the excreta was the highest (787.26 kg x hm(-2)) in Beijing, followed by in Tianjin (515.31 kg x hm(-2)) and Shanghai (505.35 kg x hm(-2)), where the environmental risk could be more serious.